Most devices, however, are adapted for 96-well or 384 well plates.
LightCycler® 2.0 accommodates only 32 samples, while LightCycler®1536 can hold up to 1536 samples. These systems spin the samples in a temperature controlled chamber, thereby achieving thermal uniformity.
BIO RAD PCR PLATES SERIES
Quiagen with Rotor-Gene series and Roche with LightCycler® 2.0, came up with a different solution, innovating the design of the apparatus to a form of a carousel. Bio-Rad, in its CFX series, incorporated six independent thermal electric modules to monitor each sample during all phases of the run. There are many approaches to overcome this problem. This construction is prone to “edge effects”, achieving different temperatures in samples located on the periphery compared to the center of the block. Most systems use sample blocks with Peltier elements that accommodate tubes, tube strips or microtiter plates. The design of the rtPCR apparatus comes in different forms. At present, however, an optimally configured rtPCR device meets the needs of most laboratories for day-to-day genomic applications. One of the first considerations is whether one should employ an endpoint PCR, q(rt)PCR or a digital PCR device. A user can choose among many available options according to research goals, throughput needs and financial capacity.
A single PCR model cannot accommodate all the needs of the research community. Table 1 lists the commonly used models and some of their important characteristics, some of the models are also discussed in detail below. The global market of these devices has substantially grown in the past three decades with a large number of models with various characteristics. PCR machines are a must-have in any life science research lab today.
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